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    當前位置 ： Millipore >>> Millipore/17-497 | Ras Activation ELISA ASSAY Kit/17-497/96 assays
    Millipore/17-497 | Ras Activation ELISA ASSAY Kit/17-497/96 assays
    • Millipore/17-497 | Ras Activation ELISA ASSAY Kit/17-497/96 assays

    Millipore/17-497 | Ras Activation ELISA ASSAY Kit/17-497/96 assays

    價格: ￥9420.00 市場價: 15700.00
    打印產品信息
    貨號: 17-497
    品牌: Millipore
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      • Description
        CatalogueNumber17-497
        Replaces17-424
        BrandFamilyUpstate
        TradeName
        • Upstate
        DescriptionRasActivationELISAASSAYKit
        BackgroundInformationRasproteinsfunctionasGDP/GTP-regulatedbinaryswitchesinsignaltransductioncascadesthatcanleadtocellgrowth,proliferation,differentiation,orsurvival.ThisRassuperfamilyhasatleastfivemajorbranchesthatincludeRas,Rho,Ran,Arf/Sar,Rab.Rasitselfhas3primaryisoforms(H-Ras,N-Ras,andK-Ras)thatdifferintheirapproximately20C-terminalaminoacids.TheyarecollectivelyreferredtoasRas.Initsactiveform,RasisboundtoGTP.Thiscausesaconformationalchangethatallowittointeractandbindtoseveraleffectormolecules,mostnotablythemembersoftheRaffamily,theRalGDSfamily,andPhosphoinositide3-kinases(PI3Kinase).RasthencleavesGTPtoGDPresultinginitsinactivation.Initsoncogenic,mutatedstate,RasisunabletohydrolyzeGTPtoGDP,thusstayinginanactivestateandactivatingnumerouspathways.
        Duetoitsroleasakeyregulatorofcellularfunctionsanditsimplicationsinvariouscancers,Rashasbeenapopulartargetforcancerresearchandanti-cancertherapeuticsforthepasttwodecadesforbothacademicandpharmaceuticalresearch.Itwasalsothefirsthumanoncogeneidentified.MutationsincellularRashavebeenfoundtobepresentinalargepercentageofallhumancancers.Morespecifically,K-Rasmutationsoccurfrequentlyinlung,pancreatic,andcoloncancers,whereasH-Rasmutationsareprevalentinbladder,kidney,andthyroidcancers,andN-Rasmutationsareassociatedwithmelanoma,hepatocellularcarcinoma,andleukemia(3).Inrecentstudies,RasactivityhasalsobeenimplicatedinanumberofhumandevelopmentdefectssuchasCostellosyndromeandNoonansyndrome(4).
        OnepaththatthepharmaceuticalindustryhastakentocontrolRasanditsactivityisbyattackingwhatsomeconsideritsAchilles’heel.Foritsactivation,Rasmustlocalizetotheplasmamembrane,butinterestingly,itlacksatransmembranedomain.Toachievethis,Rasmustfirstundergoapost-translationalmodificationknownasprenylationorgeranylationatitsC-terminalCAAXmotif.ThesedrugshaveyettopassclinicaltrialsthoughandthereisdoubtthattheywilleverbesuccessfulintreatingtumorsassociatedwithRasactivation.
        MaterialsRequiredbutNotDelivered1.Multi-channelorrepeatingPipettes
        2.Plateshakeratroomtemperatureandat4oC.
        3.Pipettors&tipscapableofaccuratelymeasuring1-1000μL
        4.Graphingsoftwareforplottingdataorgraphpaperformanualplottingofdata
        5.Multi-channelpipettorreservoirs
        6.Proteaseinhibitors
        7.Nucleasefreewater/deionizedwater
        8.PBS
        9.LuminometerorCCDcameracoupledimagingsystem
        ProductInformation
        Components
        • 1.Glutathione-CoatedPlate,96-wellstripformat:(PartNo.3007063)1platecontaining12x8strip-wellscoatedwithglutathione.
        • 2.Anti-RasDetectionAntibody:(PartNo.2006992)Onevialcontaining10μLofanti-Rasmousemonoclonalprimaryantibody.CanspecificallydetectK-,H-,N-isoformsofRas.
        • 3.Mg2+Lysis/WashBuffer,5X:(PartNo.CS202617)1vialcontaining18mLof5XMg2+Lysis/WashBufferforpreparationofcelllysate.
        • 4.20%Tween?20:(PartNo.#20-246)Onevialcontaining3mLof20%Tween?20.
        • 5.10%BSAinTBS(BlockingBuffer):(PartNo.20-191)Onebottlecontaining10mL10%BSAin1XTBS.
        • 6.20XTBSWash:(PartNo.20-190)Onebottlecontaining50mLof20XTBS,pH7.4.
        • 7.GtxMs,HRP:(PartNo.CS202619)Oneambervialcontaining25μLofgoatanti-mouseHRPconjugatedantibody.
        • 8.ChemiluminescentReactionBuffer:(PartNo.90496)Oneamberbottlecontaining8mLofChemiluminescentReactionBuffer.Keepoutoflight.
        • 9.ChemiluminescentDetectionReagent:(PartNo.90495)Onebottlevialcontaining4mLofChemiluminescentDetectionReagent.Keepoutoflight.
        • Storeat-80oC
        • 10.Raf-1-RBD,GST:(PartNo.14-863)2vialscontaining150μLofRaf-1-RBDat2μg/μL.
        • 11.EGFstimulatedHeLaCellLysate:(PartNo.12-500)2vialscontaining40μLoflysateat5mg/mLconcentration,lysedinMg2+Lysis/Washbuffer(Catalog#CS202617).
        StorageandShippingInformation
        Applications
        KeyApplications
        • ELISA
        • CellFunctionAssay
        BIOLOGicalInformation
        SpeciesReactivity
        • Human
        • Mouse
        • Rat
        AnalytesAvailable
        • Ras
        EntrezGeneNumber
        EntrezGeneSummaryK-Ras
        Thisgene,aKirstenrasoncogenehomologfromthemammalianrasgenefamily,encodesaproteinthatisamemberofthesmallGTPasesuperfamily.AsingleaminoacidsubstitutionisresponsIBLeforanactivatingmutation.Thetransformingproteinthatresultsisimplicatedinvariousmalignancies,includinglungadenocarcinoma,mucinousadenoma,ductalcarcinomaofthepancreasandcolorectalcarcinoma.AlternativesplicingleadstovariantsencodingtwoisoformsthatdifferintheC-terminalregion.[providedbyRefSeq]
        H-Ras
        ThisgenebelongstotheRasoncogenefamily,whosemembersarerelatedtothetransforminggenesofmammaliansarcomaretroviruses.Theproductsencodedbythesegenesfunctioninsignaltransductionpathways.TheseproteinscanbindGTPandGDP,andtheyhaveintrinsicGTPaseactivity.Thisproteinundergoesacontinuouscycleofde-andre-palmitoylation,whichregulatesitsrapidexchangebetweentheplasmamembraneandtheGolgiapparatus.MutationsinthisgenecauseCostellosyndrome,adiseasecharacterizedbyincreasedgrowthattheprenatalstage,growthdeficiencyatthepostnatalstage,predispositiontotumorformation,mentalretardation,skinandmusculoskeletalabnormalities,distinctivefacialappearanceandcardiovascularabnormalities.Defectsinthisgeneareimplicatedinavarietyofcancers,includingbladdercancer,follicularthyroidcancer,andoralsquamouscellcarcinoma.Multipletranscriptvariants,whichencodedifferentisoforms,havebeenidentifiedforthisgene.[providedbyRefSeq]
        GeneSymbol
        • KRAS
        • K-Ras
        • v-Ki-ras2Kirstenratsarcomaviraloncogenehomolog
        • K-rasp21protein
        • HRAS
        • H-Ras
        • Ha-Ras
        • NRAS
        • N-Ras
        • neuroblastomaRASviral(v-ras)oncogenehomolog
        UniProtNumber
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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